Mar 09, 2020 given this, it is paramount to have an objective method with which the quality of energy seals can be evaluated. Phaseresolved optical coherence tomography and optical. High speed, wide velocity dynamic range doppler optical. A comparison of doppler optical coherence tomography methods. Sigal1,2, zach nadler1, andrew francis1, michelle g. Spectroscopic doppler analysis for visiblelight optical. Selfreferenced doppler optical coherence tomography. Since its introduction in the early 1990s it has continuously improved in. System design, signal processing, and performance victor x. Osa multiple and dependent scattering effects in doppler. Measuring retinal blood flow in rats using doppler optical. Realtime phaseresolved optical coherence tomography and optical doppler tomography zhihua ding, yonghua zhao, hongwu ren, j.
In vivo imaging of human retinal flow dynamics by color. Optical coherence tomographyprinciples and applications 241 contents page 1. Doppler optical coherence tomography is an extension of oct, where it combines the doppler effect principle to achieve high resolution tomographic images in biological tissues. In spectral domain optical coherence tomography sdoct, any transverse motion component of a detected obliquely moving. Gordon, emily sengyue3, stewart lo3, bing qi 3, julius pekar 3, alvin mok, brian c. Optical coherence tomography oct 5,6 is a noninvasive technique which can optically resolve threedimensional structures with sensitivity to types of flow within the images. Nelson, phaseresolved optical coherence tomography and optical doppler tomography for imaging blood flow in human skin with fast scanning speed and high velocity sensitivity. This chapter describes doppler optical coherence tomography doct. Doppler optical coherence tomography oct is widely used for measuring retinal blood flow. En face projection of doppler oct images clearly showed rams at the corresponding. The phase information can be extracted for functional imaging, such as doppler imaging or polarization sensitive imaging 5, 6. In this paper we demonstrate a new algorithm for use in doppler optical coherence tomography doct to allow the detection of flow with a peak velocity of over 1. Apr 29, 2015 closed loop tracked doppler optical coherence tomography based heart monitor for the drosophila.
Analogous to doppler ultrasound, doppler oct doct or optical doppler tomography odt is a functional extension of oct which combines the doppler principle with oct 57. Optical coherence tomography, or oct, is a technique for obtaining subsurface images of translucent or opaque materials at a resolution equivalent to a lowpower microscope. Interstitial doppler optical coherence tomography as a local. S0034488503187039 optical coherence tomographyprinciples and applications a f fercher 1, w drexler, c k hitzenberger and t lasser2 1 institute of medical physics, university of vienna, waehringer strasse, a1090 wien, austria 2 laboratoire doptique biomedicale, institut d. All the methods are able to quantify flow speed when the flow rate is within a certain range, which is dependent on the adjacent aline. Institute of physics publishing reports on progress in physics rep. Doppler optical coherence tomography doct or optical doppler tomography odt is one kind of functional extension of oct which combines the doppler principle with oct and provides invivo functional imaging of moving samples, flows and moving constituents in biological tissues.
Closed loop tracked doppler optical coherence tomography based heart monitor for the drosophila. A heartbeat trace of freely moving larva bottom was obtained by a low coherence interferometry based doppler sensing technique. Spectral domain optical coherence tomography sdoct7 is. Recently, cdoct was adapted to a slitlamp biomicroscope for imaging structure and blood flow in the human retina. Pdf doppler optical coherence tomography for energy seal. Optical coherence tomography oct is a noninvasive optical imaging modality that provides micronscale resolution of threedimensional 3d tissue morphology 1. We have tested the feasibility of realtime localized blood flow measurements, obtained with interstitial is doppler optical coherence tomography doct, to predict photodynamic therapy pdtinduced tumor necrosis deep within solid dunning rat prostate tumors. However, variations in the axial length can create a bias unaccounted for in. Spectral contrast optical coherence tomography angiography. Oct 01, 2012 analogous to doppler ultrasound, doppler oct doct or optical doppler tomography odt is a functional extension of oct which combines the doppler principle with oct 57.
View the article pdf and any associated supplements and figures for a period of 48 hours. Optical coherence tomography is based on lowcoherence interferometry, typically employing near. Doppler optical coherence tomography doct is arguably the most widely used form of functional oct where additional information is obtained beyond the inherent contrast due to variations in index of refraction. Existing doppler oct methods require the eyeball axial length, in which empirical values are usually used. A method for generating a velocityindicating, tomographic image of a sample in an optical coherence tomography system includes the steps of a acquiring crosscorrelation data from the interferometer.
Lin, alexander j tromberg, bruce j chen, zhongping abstract. Technological developments pushed the performance of oct by several orders of magnitude. Using this strategy, we reduced commonmode interferometer noise to. Interstitial doppler optical coherence tomography as a. A comparison of doppler optical coherence tomography methods gangjun liu,1,2, alexander j. Doppler optical coherence tomography doct allows simultaneous micrometerscale resolution crosssectional imaging of tissue structure and blood flow. Doppler optical coherence tomography oct is a technique to image tissue morphology and to measure flow in turbid media. Phaseresolved doct is one of the preferred doct methods to extract the doppler frequency because of its high velocity sensitivity 8,9. Given this, it is paramount to have an objective method with which the quality of energy seals can be evaluated. High speed, wide velocity dynamic range doppler optical coherence tomography part ii. Eye movements are measured by the pooled standard deviation of the inner limiting membrane and the maximum difference between two frames that is estimated by. Phase unwrapping for doppler spectral domain optical. Variations in tissue scattering due to inhomogeneities in the optical index of refraction provide imaging contrast. A methodology employing doppler optical coherence tomography doct for the evaluation of energy seals is introduced.
Optical coherence tomography oct is a noninvasive, high resolution imaging modality capable of capturing threedimensional images from highly scattering biological tissue. Gordon, bing qi 3, julius pekar, stuart lo3, emily sengyue 3, alvin mok, brian c. We would like to thank many of our colleagues who have contributed to the doppler oct project at the beckman laser institute and department of biomedical engineering at uci, particularly the students and postdoctoral fellows. Multiple and dependent scattering effects in doppler optical. Current methodologies used for this purpose can be problematic in the evaluation of small vessel seals. Four eyes of four patients with ruptured rams were prospectively studied. To describe doppler optical coherence tomography oct imaging of ruptured retinal arterial macroaneurysms rams. Optical coherence tomography oct 1 and its doppler variants, optical doppler tomography odt 2 or color doppler optical coherence tomography cdoct 3, are analogous to ultrasound imaging where light is used instead of sound. We propose a phase unwrapping method which is suitable for correcting phase in doppler oct images.
Also explore the seminar topics paper on optical coherence tomography with abstract or synopsis, documentation on advantages and disadvantages, base paper presentation slides for ieee final year ieee biomedical engineering, biotechnology in btech, be, mtech students for the year 2015 2016. High speed, wide velocity dynamic range doppler optical coherence tomography part i. For intralipid solutions with varying scattering strength, the effect of multiple and dependent. Spectral calibration for spectral domain optical coherence. Optical imaging has become an important tool in neuroscience research. Flow measurement by lateral resonant doppler optical coherence. View the article pdf and any associated supplements and figures. It is used for medical imaging and industrial nondestructive testing ndt. Pdf twodimensional velocity estimation for doppler. Not only conserving the excellence of oct, doppler optical coherence tomography also combines the doppler effect principle as a whole. Doppler optical coherence tomography as a promising tool.
Mobility and transverse flow visualization using phase. As such doppler oct has the potential to improve our abilities to diagnose and monitor ocular vascular diseases. Doppler optical coherence tomography doct is a noninvasive optical diagnostic technique, which is well suited for the quantitative mapping of microflow. Segmentation of doppler optical coherence tomography signatures using a supportvector machine amardeep s. Doppler optical coherence tomography oct offers additional flow velocity information, which extends the application of oct. Imaging in vivo cardiac dynamics of xenopus laevis victor x. Similar to the acoustic doppler effect, the speed of a. This is an imaging modality that combines doppler principles with optical coherence tomography to image tissue structure and. The basic phenomenon of doppler oct can be explained below. Investigating spontaneous retinal venous pulsation using.
Doppler optical coherence tomography for microcirculation. Vascular imaging was obtained using sweptsource doppler oct, and compared with indocyanine green angiography images. The aim of this feasibility study was to assess the clinical feasibility of an endoscopic doppler oct edoct system in the human gi. Among different configurations of oct, spectral domain oct sdoct 2, 3 can acquire images with stable phases. Optical coherence tomography oct has become a gold standard in ophthalmic imaging. Previous doppler estimation methods have utilized a transverse kasai tk. Previously, we reported a doppler optical coherence tomography doct system design 1 for highspeed imaging up to 32 frames per second, operating at 1. Pdf twodimensional velocity estimation for doppler optical.
It is effectively optical ultrasound, imaging reflections from within tissue to provide crosssectional images. En face projection of doppler oct images clearly showed. Optical coherence tomography seminar report, ppt, pdf for. Pdf turbulence monitoring with doppler optical coherence. However, for highly scattering and dense media multiple and concentration dependent scattering can occur. The most promising candidate is doppler optical coherence tomography doct, which shares the advantage with laser doppler technology of being noncontact, labelfree, and without employing hazardous radiation. Pdf optical coherence tomography oct has revolutionized ophthalmology. High speed microstructural oct imaging has been demonstrated at various pixelresolution and frame. Phase wrapping is the inherent problem that limits measureable range of doppler oct. Heartbeatphasecoherent doppler optical coherence tomography for measuring pulsatile ocular blood flow. Endoscopic doppler optical coherence tomography in the.
Doppler optical coherence tomography, in optical coherence tomography. Gold nanorods as a contrast agent for doppler optical. Optical coherence tomographyprinciples and applications. A comparison of doppler optical coherence tomography. The raw oct images, including both doppler and intensity images, are first tested for image quality using doppler optical coherence tomography of retinal circulation doctorc software.
Izatt department of biomedical engineering, duke university, durham, north carolina 27708 received june 25, 2002 doppler optical coherence tomography doct allows simultaneous micrometerscale resolution crosssectional imaging of tissue structure and blood flow. Gold nanorods as a contrast agent for doppler optical coherence tomography bo wang1,2,3, larry kagemann1,2, joel s. Doppler optical coherence tomography sciencedirect. We demonstrate a fiberoptic polarizationdiversitybased differential phase contrast doct system as a method to perform selfreferenced velocimetry in highly scattering media. Leitgeb group of biomedical optics, center of medical physics and biomedical engineering, medical university of vienna, waehringerstrasse, 1090 vienna, austria rainer. Isdoct was used to quantify the pdtinduced microvascular shutdown rate in s. Us6006128a doppler flow imaging using optical coherence. Closed loop tracked doppler optical coherence tomography. Doppler optical coherence tomography of retinal circulation. Doppler optical coherence tomography for energy seal evaluation and comparison to visual evaluation article pdf available in journal of biomedical optics 2503. Since the first demonstration of doppler optical coherence tomography oct in 1997, several functional extensions of doppler oct have been. Optical coherence tomography oct has a microresolution with a penetration depth of about 2 mm and field of view of about 10 mm.
Segmentation of doppler optical coherence tomography. In the past few years, due to the development of fast swept laser sources and high speed detector arrays, fourier domain oct systems have experienced dramatic improvement compared with the time. We propose a simple method of linearizing spectral interference fringes of spectral domain optical coherence tomography based on bscan doppler frequency shift dfs, which can be obtained by offsetting the laser beam from the pivot of a scanning mirror. Selfreferenced doppler optical coherence tomography siavash yazdanfar and joseph a. Optical coherence tomography is a micrometerscale imaging modality that permits. Turbulence monitoring with doppler optical coherence tomography. However, variations in the axial length can create a bias unaccounted for in the retinal blood flow measurement. Background color doppler optical coherence tomography cdoct combines laser doppler velocimetry and optical coherence tomography for simultaneous micronscale resolution crosssectional imaging of tissue microstructure and blood flow. Development and applications 4 nowadays many others light properties are explored too, like polarization sensitive oct or doppler shift oct are already established, these techniques can extract information about fiber alignment or particles velocity within the sample, respectively. Pdf doppler optical coherence tomography researchgate. Phaseresolved doct is one of the preferred doct methods to extract the doppler frequency because of its high velocity sensitivity 8, 9. Sdoct measures flow velocity by detecting the phase difference between two sequential alines. Realtime phaseresolved optical coherence tomography and. Optical coherence tomography oct is a recently discovered technique, which displays images of the tissue by using the backscattered light.
We compare, in detail, the phaseresolved color doppler prcd, phaseresolved doppler variance prdv and intensitybased doppler variance ibdv methods. Optical doppler tomography odt,14 also termed doppler optical coherence tomography, is a recently developed optical technique for imaging both the tissue structure and the flow velocity of moving par. Contrary to ldv, doct provides, however, fully quantitative volumetric information about blood flow together with the vascular and. In its most basic form, it is based on single mie scattering. Doppler optical coherence tomography for energy seal. Doppler optical coherence tomography in determination of.
A short matlabbased tutorial on performing doppler and dynamic light scattering processing on optical coherence tomography. Doppler optical coherence tomography for microcirculation studies. Optical coherence tomography oct is a recently developed imaging modality based on coherencedomain optical technology 14. Osa selfreferenced doppler optical coherence tomography. Doppler optical coherence tomography as a promising tool for. Advances in doppler optical coherence tomography and. Phaseresolved doppler optical coherence tomography 43 2. Optical coherence tomography oct is an imaging technique that uses lowcoherence light to capture micrometerresolution, two and threedimensional images from within optical scattering media e. The tutorial aims to give basic physical intuition to the measurements. This makes oct well suited for analyzing the anatomical and internal structural assessment of the middle ear.
Noninvasive vascular imaging of ruptured retinal arterial. Doppler optical coherence tomography for microcirculation studies a thesis submitted to the university of manchester for the degree of doctor of philosophy in the. Doppler optical coherence tomography as a promising tool for detecting fluid in the human middle ear 3d local environment live imaging for single cell protein analysis of bone tissuelive imaging is a novel methodological platform for linking individual expression profiles of cells within bone tissues to their local remodeling and mechanical. Oct uses coherence gating of backscattered light for tomographic imaging of tissue structure.
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